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methocult h4100 (methylcellulose medium)  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc methocult h4100 (methylcellulose medium)

    Methocult H4100 (Methylcellulose Medium), supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/methylcellulose+methocult+h4100/methocult/pmc11964678-74-0-5
    Average 90 stars, based on 1 article reviews
    methocult h4100 (methylcellulose medium) - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Lymphopoiesis is attenuated upon hepatocyte-specific deletion of the cytochrome c oxidase assembly factor Sco1"

    Article Title: Lymphopoiesis is attenuated upon hepatocyte-specific deletion of the cytochrome c oxidase assembly factor Sco1

    Journal: iScience

    doi: 10.1016/j.isci.2025.112151


    Figure Legend Snippet:

    Techniques Used: Control, Enzyme-linked Immunosorbent Assay, Functional Assay, Western Blot, Recombinant, Protease Inhibitor, Diagnostic Assay, Staining, Software

    Related Articles

    FACS:

    Article Title: Fetal Muse-based therapy prevents lethal radio-induced gastrointestinal syndrome by intestinal regeneration.
    Article Snippet: Bulk production of Muse cell derived‐clusters by using methylcellulose gel To avoid cell adherence, plates were pre-coated with a 3% poly-HEMA solution in 95% Ethanol [poly(2hydroxyethyl methacrylate), #P3932, Sigma-Aldrich]. .. FACS-sorted Muse cells were mixed to Low-glucose DMEM + GlutaMAX medium containing 10% FBS and 0.9% methylcellulose (MethoCult H4100, #04100, StemCell Technologies). ..

    Colony Assay:

    Article Title: Z-Ligustilide Selectively Targets AML by Restoring Nuclear Receptors Nur77 and NOR-1-mediated Apoptosis and Differentiation.
    Article Snippet: Background: Acute myeloid leukemia (AML) is a devastating hematologic malignancy with a high mortality.. The nuclear receptors Nur77 and NOR-1 are commonly downregulated in human AML blasts and have emerged as key therapeutic targets for AML.. Methods: This study aimed to identify Z-ligustilide (Z-LIG), the main phthalide of Rhizoma Chuanxiong, as a potential agent that can selectively target AML.

    other:

    Article Title: Norchelerythrine from Corydalis incisa (Thunb.) Pers. promotes differentiation and apoptosis by activating DNA damage response in acute myeloid leukemia.
    Article Snippet: Acute myeloid leukemia (AML) is the most preva‐ lent form of leukemia in adults.. The cornerstone of first‐line chemotherapy for AML has poor survival rates, underscoring the urgent need for development of novel therapeutic agents.. Differentiation therapy targets the blockade of differentiation in myeloid progenitor cells.

    Article Title: Plasma Cells are Obligate Effectors of Enhanced Myelopoiesis in Aging Bone Marrow
    Article Snippet: MC medium was prepared by supplementing α-MEM with methylcellulose (1%, MethoCult H4100, STEMCELL Technologies), heat-inactivated fetal calf serum (30%), 2-ME (50 μM), Penicillin-Streptomycin (100 U/mL), L-glutamine (2 mM), Gentamicin (50 μg/mL), sodium pyruvate (1 mM), non-essential amino acids (1×) and vitamins (1×), SCF (50 ng/mL), IL-3 (30 ng/mL), IL-6 (10 ng/mL), IL-11 (10 ng/mL) and GM-CSF (20 ng/mL).

    Clonogenic Assay:

    Article Title: Co-Administration of Fendiline Hydrochloride Enhances Chemotherapeutic Efficacy of Cisplatin in Neuroblastoma Treatment
    Article Snippet: .. A medium consisting of DMEM with 0.4% methylcellulose (Methocult H4100, StemCell Technologies, Vancouver, BC, Canada) supplemented with 10% FBS, 100 U/mL penicillin/streptomycin, and 2 mM L-glutamine was used to perform clonogenic assay. ..



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    a , b Representative images of colonies grown for 14 days in <t>methylcellulose</t> from CD34+ HSPC sorted from COVID− and COVID+S samples. Photographs ( a ) and pictures taken at ×4 magnification ( b ) of representative 35-mm dishes are shown. Scale bar: 500 μm. c – e Quantification of colony-forming units for granulocytes, erythrocytes, monocytes, megakaryocytes (CFU-GEMM) ( c ), burst-forming units-erythroid and colony-forming units-erythroid (BFU-E/CFU-E) ( d ), and of colony-forming units for granulocytes and macrophages (CFU-GM) ( e ) in COVID− ( n = 3 patients) and COVID+S ( n = 3 patients) samples. Data are presented as means with SEM and individual values. Statistical analyses were performed with two-tailed unpaired t test ( d ) or two-tailed unpaired t test with Welch’s correction ( c , e ). ns not significant; * P value < 0.05. Source data are available in Supplementary Data .
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    a , b Representative images of colonies grown for 14 days in <t>methylcellulose</t> from CD34+ HSPC sorted from COVID− and COVID+S samples. Photographs ( a ) and pictures taken at ×4 magnification ( b ) of representative 35-mm dishes are shown. Scale bar: 500 μm. c – e Quantification of colony-forming units for granulocytes, erythrocytes, monocytes, megakaryocytes (CFU-GEMM) ( c ), burst-forming units-erythroid and colony-forming units-erythroid (BFU-E/CFU-E) ( d ), and of colony-forming units for granulocytes and macrophages (CFU-GM) ( e ) in COVID− ( n = 3 patients) and COVID+S ( n = 3 patients) samples. Data are presented as means with SEM and individual values. Statistical analyses were performed with two-tailed unpaired t test ( d ) or two-tailed unpaired t test with Welch’s correction ( c , e ). ns not significant; * P value < 0.05. Source data are available in Supplementary Data .
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    Image Search Results


    Journal: iScience

    Article Title: Lymphopoiesis is attenuated upon hepatocyte-specific deletion of the cytochrome c oxidase assembly factor Sco1

    doi: 10.1016/j.isci.2025.112151

    Figure Lengend Snippet:

    Article Snippet: MethoCult H4100 (methylcellulose medium) , STEMCELL Technologies , Cat# 04100.

    Techniques: Control, Enzyme-linked Immunosorbent Assay, Functional Assay, Western Blot, Recombinant, Protease Inhibitor, Diagnostic Assay, Staining, Software

    a , b Representative images of colonies grown for 14 days in methylcellulose from CD34+ HSPC sorted from COVID− and COVID+S samples. Photographs ( a ) and pictures taken at ×4 magnification ( b ) of representative 35-mm dishes are shown. Scale bar: 500 μm. c – e Quantification of colony-forming units for granulocytes, erythrocytes, monocytes, megakaryocytes (CFU-GEMM) ( c ), burst-forming units-erythroid and colony-forming units-erythroid (BFU-E/CFU-E) ( d ), and of colony-forming units for granulocytes and macrophages (CFU-GM) ( e ) in COVID− ( n = 3 patients) and COVID+S ( n = 3 patients) samples. Data are presented as means with SEM and individual values. Statistical analyses were performed with two-tailed unpaired t test ( d ) or two-tailed unpaired t test with Welch’s correction ( c , e ). ns not significant; * P value < 0.05. Source data are available in Supplementary Data .

    Journal: Communications Medicine

    Article Title: Enhanced fetal hematopoiesis in response to symptomatic SARS-CoV-2 infection during pregnancy

    doi: 10.1038/s43856-023-00406-6

    Figure Lengend Snippet: a , b Representative images of colonies grown for 14 days in methylcellulose from CD34+ HSPC sorted from COVID− and COVID+S samples. Photographs ( a ) and pictures taken at ×4 magnification ( b ) of representative 35-mm dishes are shown. Scale bar: 500 μm. c – e Quantification of colony-forming units for granulocytes, erythrocytes, monocytes, megakaryocytes (CFU-GEMM) ( c ), burst-forming units-erythroid and colony-forming units-erythroid (BFU-E/CFU-E) ( d ), and of colony-forming units for granulocytes and macrophages (CFU-GM) ( e ) in COVID− ( n = 3 patients) and COVID+S ( n = 3 patients) samples. Data are presented as means with SEM and individual values. Statistical analyses were performed with two-tailed unpaired t test ( d ) or two-tailed unpaired t test with Welch’s correction ( c , e ). ns not significant; * P value < 0.05. Source data are available in Supplementary Data .

    Article Snippet: To perform colony cultures, 1000 cells/ml per condition of CD34+ progenitors were seeded in methylcellulose medium (H4100 Methocult media, StemCell Technologies) supplemented with 12.5% bovine serum albumin (Sigma-Aldrich) (neutralized with 7.5% sodium bicarbonate (Sigma-Aldrich)), 30% fetal bovine serum, 1 mM 2-mercaptoethanol (Sigma-Aldrich), 1% l -glutamine (Gibco), and in the presence of a cocktail of recombinant cytokines containing 1 U/mL EPO, 20 ng/mL TPO, 25 ng/mL SCF, 10 ng/mL IL-3, 10 ng/mL FLT3-L, 20 ng/mL G-CSF, 10 ng/mL IL-6 (all cytokines were purchased from Pepro Tech).

    Techniques: Two Tailed Test